Development of an in vitro translation-based screening system to identify mRNA translation inhibitors
The goal of our project is to develop a platform that allows the identification of small molecules that inhibit translation in human cells using cell-free lysates. The lysates are also used for studying the mode of action of proteins from coronaviruses (2,3).
With the funding that was acquired thanks to the UniBern Forschungsstiftungs grant we obtained a shaking incubator device that allows us to produce ample amounts of cells and lysates for our screening purposes, based on a previously published protocol (1).
Evangelos D. Karousis, PhD
Dept. of Chemistry, Biochemistry and Pharmaceutical Sciences
